產(chǎn)品分類
PH7.2磷酸鹽緩沖液配制方法
更新時(shí)間：2013-03-09 瀏覽次數(shù)：5571
Microbiologics公司的部分菌株產(chǎn)品有自帶的稀釋液，有些就需要自制的PH7.2磷酸鹽緩沖液，實(shí)驗(yàn)室也有很多地方會(huì)用到該緩沖液，其配制方法如下：
Stock Solution— Dissolve 34 g of monobasic potassium phosphate in about 500 mL of water contained in a 1000-mL volumetric flask. Adjust to pH 7.2 ± 0.1 by the addition of sodium hydroxide TS （about 175 mL）, add water to volume, and mix. Dispense and sterilize. Store under refrigeration.
For use, dilute the Stock Solution with water in the ratio of 1 to 800, and sterilize.